The technique involves the transfer of electrophoresed DNA from the electrophoresis gel to a nitrocellulose membrane. The DNA fragments are separated based on their size and charge during electrophoresis. Southern blotting is based on the principle of separation of DNA fragments by gel electrophoresis followed by the identification by labeled probe hybridization. In addition to its use for DNA analysis, the immunologists have long used the southern blotting for gene identification in somatic rearrangements and transgene studies. Furthermore, southern blotting is used to identify methylated sites in particular genes. Southern blotting could be used for homology-based cloning by exploring the amino acid sequence of the protein product of the gene of interest. Sequences hybridizing with the hybridization probe are further analyzed to obtain the full-length sequence of the gene of interest. These oligonucleotides are chemically synthesized, radiolabeled, and then used for the cloned DNA fragments. Oligonucleotides, similar to the target sequence are designed. After immobilization, the DNA fragments could be subjected to hybridization analysis, enabling the bands with sequence similarity to a labeled probe. The method involves the transfer of DNA fragments from an electrophoresis gel to a membrane causing immobilization of the DNA fragments and bands are produced. Southern, who developed the technique in the 1970s. Southern blotting is a hybridization technique used for deoxyribose nucleic acid (DNA). Balances, Scales and Weighing Equipment.Get the free view of Chapter 4, Molecular Basis of Inheritance Biology 12th Standard HSC for Maharashtra State Board additional questions for Mathematics Biology 12th Standard HSC for Maharashtra State Board Maharashtra State Board,Īnd you can use to keep it handy for your exam preparation. Maximum Maharashtra State Board Biology 12th Standard HSC for Maharashtra State Board students prefer Balbharati Textbook Solutions to score more in exams. The questions involved in Balbharati Solutions are essential questions that can be asked in the final exam. Using Balbharati Biology 12th Standard HSC for Maharashtra State Board solutions Molecular Basis of Inheritance exercise by students is an easy way to prepare for the exams, as they involve solutionsĪrranged chapter-wise and also page-wise. Balbharati textbook solutions can be a core help for self-study and provide excellent self-help guidance for students.Ĭoncepts covered in Biology 12th Standard HSC for Maharashtra State Board Maharashtra State Board chapter 4 Molecular Basis of Inheritance are Deoxyribonucleic Acid (DNA) and Its Structure, The Genetic Material is a DNA, DNA Replication, Protein Synthesis, Regulation of Gene Expression, Operon Concept, Genomics, Human Genome Project, DNA Fingerprinting Technique, Packaging of DNA Helix. This will clear students' doubts about questions and improve their application skills while preparing for board exams.įurther, we at provide such solutions so students can prepare for written exams. The detailed, step-by-step solutions will help you understand the concepts better and clarify any confusion.īalbharati solutions for Mathematics Biology 12th Standard HSC for Maharashtra State Board Maharashtra State Board 4 (Molecular Basis of Inheritance) include all questions with answers and detailed explanations. has the Maharashtra State Board Mathematics Biology 12th Standard HSC for Maharashtra State Board Maharashtra State Board solutions in a manner that help students
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